All-optical Control of Brain Functioning with Optogenetics and Multi-Photon Microscopy - ENGLISH Course Présentiel
Dernière mise à jour : 01/10/2025
Description
AXE 10 - Microscopie et imagerie
Scientific and technical environment of the training course
Microscopie photonique
All-optical Control of Brain Functioning with Optogenetics and Multi-Photon Microscopy
COURSE DIRECTORS
- Valentina EMILIANI - Senior researcher - UMR 7210
- Eirini PAPAGIAKOUMOU - Researcher - UMR 7210
LOCATION: PARIS (75)
ORGANISATION: 8 days from 09:00 am to 06:00 pm. Training course in English. 15 attendees maximum. The next session will take place in March 2025. Registration will open in November 2024. Premises accessible to people with reduced mobility (PRM).
TRAINING PROGRAMME:
- Lectures:
- Introduction to optogenetics
- Wave front shaping and liquid crystal matrix
- Viral vector design
- Computer generated holography
- Temporal focusing
- Three-dimensional light patterning and temporal focusing
- Two-photon optogenetics: scanning, spiral scanning, parallel illumination: examples and comparison among the different approaches
- All-optical interrogation of brain circuits
- Voltage and calcium imaging
- 2P Miniscope for two-photon imaging in freely moving mice
- Two-photon all-optical circuits manipulation in freely moving mice
- Patterned voltage and calcium imaging
- Three photon imaging and optogenetics
- Mesoscopy and large scale holography
- Practical courses (in small rotating groups of 7 attendees max with 2 trainers per group):
- How to build up a holographic optical set-up (optical design and software)
- In vitro and in vivo all-optical manipulation of neuronal circuits
- Two-photon all-optical circuits manipulation in freely moving mice
- Two-photon scanless voltage imaging
- Projects from applicants (practical): proposition of projects by the attendees and discussion on their feasibility
AT THE END OF THE TRAINING COURSE:
- Satisfaction survey from trainees
- A training certificate is delivered
Objectifs de la formation
- Learn how to choose the most suitable combination of actuators and reporters, illumination methods and targeting strategy for each specific application
- Acquire the skills and technologies required to design and build up an all-optical system for optical control of neuronal circuits
Public visé
Neurophysiologists, physicists (researchers, PhD students or engineers)
Prérequis
Attendees should have a strong background in neurosciences or in cell biology or in optical microscopy. To adapt the programme to trainees' expectations, we invite the attendees to download and fill out the survey from our web site and submit it when performing the pre-registration.
Modalités pédagogiques
Lectures (23 h), workshops (28 h) and presentations of projects (8 h)
Moyens et supports pédagogiques
Files in PDF format will be made available to the trainee.
Modalités d'évaluation et de suivi
- Satisfaction survey from trainees
- A training certificate is delivered
- A training certificate is delivered
Formateurs
Informations sur l'admission
The next session will take place in March 2025. Registration will open in November 2024.
Informations sur l'accessibilité
Premises accessible to people with reduced mobility (PRM).