All-optical Control of Brain Functioning with Optogenetics and Multi-Photon Microscopy - ENGLISH Course Présentiel
Dernière mise à jour : 25/10/2025
Description
Lectures
- Introduction to optogenetics
- Wave front shaping and liquid crystal matrix
- Viral vector design
- Computer generated holography
- Temporal focusing
- Three-dimensional light patterning and temporal focusing
- Two-photon optogenetics: scanning, spiral scanning, parallel illumination: examples and comparison among the different approaches
- All-optical interrogation of brain circuits
- Voltage and calcium imaging
- 2P Miniscope for two-photon imaging in freely moving mice
- Two-photon all-optical circuits manipulation in freely moving mice
- Patterned voltage and calcium imaging
- Three photon imaging and optogenetics
- Mesoscopy and large scale holography
Practical courses (in small rotating groups of 7 attendees max with 2 trainers per group)
- How to build up a holographic optical set-up (optical design and software)
- In vitro and in vivo all-optical manipulation of neuronal circuits
- Two-photon all-optical circuits manipulation in freely moving mice
- Two-photon scanless voltage imaging
- Projects from applicants (practical): proposition of projects by the attendees and discussion on their feasibility
Objectifs de la formation
- Learn how to choose the most suitable combination of actuators and reporters, illumination methods and targeting strategy for each specific application
- Acquire the skills and technologies required to design and build up an all-optical system for optical control of neuronal circuits
Public visé
Neurophysiologists, physicists (researchers, PhD students or engineers)
Prérequis
Attendees should have a strong background in neurosciences or in cell biology or in optical microscopy. To adapt the programme to trainees' expectations, we invite the attendees to download and fill out the survey from our web site and submit it when performing the pre-registration.
Modalités pédagogiques
Lectures (23 h), workshops (28 h) and presentations of projects (8 h)
Moyens et supports pédagogiques
Files in PDF format will be made available to the trainee.
Modalités d'évaluation et de suivi
Ongoing formative assessments throughout the course. A certificate of completion is issued at the end of the training.
Formateurs
EV
EMILIANI Valentina
Responsable scientifique
PE
PAPAGIAKOUMOU Eirini
Responsable scientifique
Modalités tarifaires spécifiques
All our training courses are VAT exempt. Enjoy exclusive volume discounts: save 5% when registering 3-4 participants, 10% for 5-6, and 20% for groups of 7 or more. CNRS employees benefit from a special 20% discount.
Informations sur l'accessibilité
Our laboratory is fully accessible for people with reduced mobility (PRM), with dedicated facilities (access, circulation, restrooms). For any further details, please share tour request via our registration form.